产品名称 Anti-DAP5抗体 参阅全部 DAP5 一抗
WB, IHC-P, ICC/IF more details
Mouse, Rat, Rabbit, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 204 of Human DAP5 (NP_001409)
A431 whole cell lysate and Cal27.
IF/ICC: HeLa cell line.
存放说明 Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 40% Glycerol, PBS, pH 7.0
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 102 kDa.
Use a concentration of 10 µg/ml.
功能 Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
组织特异性 Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
序列相似性 Belongs to the eIF4G family. Contains 1 MI domain. Contains 1 MIF4G domain. Contains 1 W2 domain.
翻译后修饰 Phosphorylation; hyperphosphorylated during mitosis.
Information by UniProt
Death associated protein 5 antibody
Death-associated protein 5 antibody
Anti-DAP5 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-DAP5 antibody (ab97302)
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was
ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - DAP5 antibody (ab97302)
Anti-DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg Predicted band size : 102 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAP5 antibody (ab97302)
Immunohistochemical analysis of paraffin-embedded Cal27, using ab97302 at 1/100 dilution
Anti-DAP5 antibody (ab97302)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"