All lanes : Anti-DAP5 antibody (ab85963) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 minutes
Immunoprecipitation - DAP5 antibody (ab85963)
1mg HeLa whole cell lysate was immunoprecipitated using 10µg ab85963. 20% of IP was loaded per lane and probed with ab85963 at 0.4µg/ml (lane 1) and a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.
Xie JD et al. Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain. J Biol Chem291:19364-73 (2016).
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