概述

  • 产品名称Anti-DAP5抗体
    参阅全部 DAP5 一抗
  • 描述
    兔多克隆抗体to DAP5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (PNFDGPAAEG QPGQKQSTTF RRLLISKLQD EFENRTRNVD VYDKRENPLL) of human DAP5 (NP_001409).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
  • 组织特异性Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
  • 序列相似性Belongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • 翻译后修饰Phosphorylation; hyperphosphorylated during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAP-5 antibody
    • Death associated protein 5 antibody
    • Death-associated protein 5 antibody
    • eIF-4-gamma 2 antibody
    • eIF-4G 2 antibody
    • eIF4G 2 antibody
    • EIF4G2 antibody
    • Eukaryotic translation initiation factor 4 gamma 2 antibody
    • IF4G2_HUMAN antibody
    • Nat1 antibody
    • Novel APOBEC-1 target 1 antibody
    • p97 antibody
    • Translation repressor NAT1 antibody
    • Translation repressor NAT1 antibody
    see all

Anti-DAP5 antibody 图像

  • Anti-DAP5 antibody (ab83358) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)

Anti-DAP5 antibody (ab83358)参考文献

ab83358 has not yet been referenced specifically in any publications.

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