Anti-DAP3抗体(ab155499)
Key features and details
- Rabbit polyclonal to DAP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DAP3抗体
参阅全部 DAP3 一抗 -
描述
兔多克隆抗体to DAP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 52-277 of Human DAP3 (Uniprot ID: P51398).
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 whole cell lysate; HeLa cells; Human gastric cancer tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/1000. Suggested antigen retrieval
using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Involved in mediating interferon-gamma-induced cell death. -
组织特异性
Ubiquitous. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 504320 Cow
- Entrez Gene: 7818 Human
- Entrez Gene: 65111 Mouse
- Entrez Gene: 295238 Rat
- Omim: 602074 Human
- SwissProt: P82922 Cow
- SwissProt: P51398 Human
- SwissProt: Q9ER88 Mouse
see all -
别名
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
see all
图片
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Anti-DAP3 antibody (ab155499) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling DAP3 with ab155499 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling DAP3 with ab155499 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab155499 尚未被引用在任何文献中。