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Synthetic peptide corresponding to residues in Human DAP12 protein (O43914).
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab124834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
|ICC/IF||1/250 - 1/500.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain section were permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4°C. The secondary antibody was Alexa Fluor® 488 donkey anti-rabbit used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab124834 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"