RabMAb

Anti-DAP12抗体[EPR5173] (ab124834)

概述

  • 产品名称Anti-DAP12抗体[EPR5173]
    参阅全部 DAP12 一抗
  • 描述
    兔单克隆抗体[EPR5173] to DAP12
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human DAP12 protein (O43914).

  • 阳性对照
    • Peripheral blood leukocytes, Human placenta and peripheral blood mononuclear cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD KD = 1.86 x 10 -10 M Learn more about KD
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 纯化说明Not purified; provided in cell supernatant and storage buffer.
  • 克隆单克隆
  • 克隆编号EPR5173
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
    • 组织特异性Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
    • 疾病相关Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
    • 序列相似性Belongs to the TYROBP family.
    • 翻译后修饰Tyrosine phosphorylated.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DAP 12 antibody
      • DAP12 antibody
      • DNAX activation protein 12 antibody
      • DNAX-activation protein 12 antibody
      • KAR-associated protein antibody
      • KARAP antibody
      • Killer activating receptor associated protein antibody
      • Killer-activating receptor-associated protein antibody
      • PLOSL antibody
      • TYOBP_HUMAN antibody
      • TYRO protein tyrosine kinase binding protein antibody
      • TYRO protein tyrosine kinase-binding protein antibody
      • TYROBP antibody
      see all

    Anti-DAP12 antibody [EPR5173] 图像

    • Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain section were permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4°C. The secondary antibody was Alexa Fluor® 488 donkey anti-rabbit used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      See Abreview

    • All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution

      Lane 1 : Peripheral blood leukocytes lysates
      Lane 2 : Human placenta lysates
      Lane 3 : Peripheral blood mononuclear lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 12 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-DAP12 antibody [EPR5173] (ab124834)参考文献

    ab124834 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Human Tissue sections (Blood vessels)
    Specification Blood vessels
    Permeabilization Yes - 0.1% TritonX in 0.1% PBS
    Fixative Formaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Aug 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"