Anti-DAP Kinase 1 (phospho S308)抗体[DKPS308] (ab10524)

概述

  • 产品名称Anti-DAP Kinase 1 (phospho S308)抗体[DKPS308]
    参阅全部 DAP Kinase 1 一抗
  • 描述
    小鼠单克隆抗体[DKPS308] to DAP Kinase 1 (phospho S308)
  • 经测试应用适用于: ELISA, IP, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1.

性能

应用

Our Abpromise guarantee covers the use of ab10524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IP
Dot blot
  • 应用说明Dot: Use at a concentration of 1 - 2 µg/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector.
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 10 ANK repeats.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.
    • 细胞定位Cytoplasm. Colocalizes with the actin filament system.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DAK1 antibody
      • DAP K1 antibody
      • DAP kinase 1 antibody
      • DAPK 1 antibody
      • DAPK antibody
      • DAPK1 antibody
      • DAPK1_HUMAN antibody
      • Death Associated Protein Kinase 1 antibody
      • Death-associated protein kinase 1 antibody
      • DKFZp781I035 antibody
      see all

    Anti-DAP Kinase 1 (phospho S308) antibody [DKPS308] (ab10524)参考文献

    ab10524 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (EAhy926 endothelial cell line, HUVECs, HEK293, HeL)
    Gel Running Conditions Reduced Denaturing (12%)
    Loading amount 20 µg
    Treatment Alkaline Phosphatase treated, TNF-alpha treated, Vanadate treated, NaF treated, H2O2 treated, Ceramide treated
    Specification EAhy926 endothelial cell line, HUVECs, HEK293, HeL
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Username

    Dr. Nima Abbasian

    Verified customer

    提交于 Jan 28 2016

    Thank you for your reply.
    Unfortunately we can not send out free trial vials. We do also not have small sample sizes of our products.
    I am very sorry for this.
    I would like to remind you, that in the testing offer explained to you in my ...

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    Thank you very much for your interest in ab10524.
    To our knowledge, ab10524 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab10524 now, test it in ICC/IF and submit feedback to us in the form of a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"