Anti-DAGLA抗体(ab81984)

概述

  • 产品名称Anti-DAGLA抗体
    参阅全部 DAGLA 一抗
  • 描述
    山羊多克隆抗体to DAGLA
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Synthetic peptide:

    C-PAKQDELVISAR

    , corresponding to C terminal amino acids 1031-1042 of Human DAGLA.

  • 阳性对照
    • Human liver lysates (WB). Mouse cerebellum (IHC) This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

性能

应用

Our Abpromise guarantee covers the use of ab81984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 115 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
  • 组织特异性Highly expressed in brain and pancreas.
  • 疾病相关Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA.
  • 序列相似性Belongs to the AB hydrolase superfamily. Lipase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C11orf11 antibody
    • Chromosome 11 open reading frame 11 antibody
    • DAGL(ALPHA) antibody
    • Dagla antibody
    • DAGLALPHA antibody
    • DGL alpha antibody
    • DGL-alpha antibody
    • DGLA_HUMAN antibody
    • Diacylglycerol lipase alpha antibody
    • KIAA0659 antibody
    • Neural stem cell derived dendrite regulator antibody
    • Neural stem cell-derived dendrite regulator antibody
    • NSDDR antibody
    • Sn1-specific diacylglycerol lipase alpha antibody
    see all

Anti-DAGLA antibody 图像

  • Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 115 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa,55 kDa. We are unsure as to the identity of these extra bands.
    Some minor background is detected and is blocked by the immunizing peptide.
  • Immunohistochemistical detection of DAGLA using ab81984 on formaldehyde fixed rat cerebellum tissue sections (cerebellum). Antigen retrieval step: heat mediated in citric acid pH6. 1%BSA as blocking agent for 10 mins@ rt°C. Primary antibody dilution 1/1000 for 2 hours. Secondary antibody: anti-sheep IgG conjugated to biotin (1/200). The pattern of immunolabelling is very similar to that obtained in mouse. However, the cell bodies of some Purkinje cells are also positive, this appears to be punctate and evenly distributed.

    See Abreview

  • Immunohistochemistical detection of DAGLA with ab81984 on formaldehyde fixed mouse cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. 1% BSA used as blocking agent for 10 mins @ rt°C. Primary antibody ab81984 incubated at 1/500 for 2 hours. Secondary antibody: anti sheep IgG conjugated to biotin (1/200). The upper image shows DAGL immunostaining with ab81984 on wildtype mouse cerebellum, the lower image shows an absence of DAGL alpha immunostaining on knockout mouse mouse cerebellum. These results are consistent with published data with other anti-DAGL alpha antibodies (http://jcb.rupress.org/cgi/content/full/163/3/463)

    See Abreview

  • ab81984 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81984 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DAGLA antibody (ab81984)参考文献

ab81984 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Tissue lysate - whole (brain)
Specification brain
Total protein in input 10 µg
Username

Ms. YA ZHOU

Verified customer

提交于 Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10)
Sample Monkey Cell lysate - other (COS 7)
Specification COS 7
Treatment TRANSFECT DAGLA
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - 0.2% triton
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (olfactory epithelium)
Loading amount 40 µg
Specification olfactory epithelium
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12.5%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2011

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Application Western blot
Sample Mouse Cell lysate - whole cell (Neural stem cell line)
Loading amount 30 µg
Specification Neural stem cell line
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (cerebellum)
Specification cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset cerebellum)
Specification Marmoset cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 15 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"