Anti-DAG1抗体(ab110543)

概述

  • 产品名称Anti-DAG1抗体
    参阅全部 DAG1 一抗
  • 描述
    兔多克隆抗体to DAG1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 721-770 (PPSPGSSAAP ATEVPDRDPE KSSEDDVYLH TVIPAVVVAA ILLIAGIIAM), of Mouse DAG1 (NP_034147)

  • 阳性对照
    • Mouse heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab110543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
    Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains, and for certain adenoviruses. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for M.leprae in peripheral nerve Schwann cells but only in the presence of the G-domain of LAMA2, and for lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses.
    Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.
  • 组织特异性Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.
  • 疾病相关Defects in DAG1 are the cause of muscular dystrophy-dystroglycanopathy limb-girdle type C7 (MDDGC7) [MIM:613818]. An autosomal recessive muscular dystrophy showing onset in early childhood, and associated with mental retardation without structural brain anomalies. Note=MDDGC7 is caused by DAG1 mutations that interfere with normal post-translational processing, resulting in defective DAG1 glycosylation and impaired interactions with extracellular-matrix components. Other muscular dystrophy-dystroglycanopathies are caused by defects in enzymes involved in protein O-glycosylation.
  • 序列相似性Contains 1 peptidase S72 domain.
  • 翻译后修饰O- and N-glycosylated. Alpha-dystroglycan is heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation of alpha-DAG1 is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE) protein and is required for laminin binding. O-mannosylation is also required for binding lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses. The O-glycosyl hexose on Thr-367, Thr-369, Thr-372, Thr-381 and Thr-388 is probably mannose. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The beta subunit is N-glycosylated.
    Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglcan can occur releasing a peptide of about 30 kDa.
    SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry.
  • 细胞定位Secreted > extracellular space and Cell membrane. Cytoplasm > cytoskeleton. Nucleus > nucleoplasm. The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In peripheral nerves, localizes to the Schwann cell membrane. Colocalizes with ERM proteins in Schwann-cell microvilli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 156DAG antibody
    • A3a antibody
    • Agrin receptor antibody
    • AGRNR antibody
    • Alpha-DG antibody
    • alpha-DG-N antibody
    • Beta-DG antibody
    • Beta-dystroglycan antibody
    • DAG antibody
    • Dag1 antibody
    • DAG1_HUMAN antibody
    • Dystroglycan 1 (dystrophin-associated glycoprotein 1) antibody
    • Dystroglycan antibody
    • Dystroglycan, alpha antibody
    • Dystrophin-associated glycoprotein 1 antibody
    • MDDGC7 antibody
    • MDDGC9 antibody
    • OTTHUMP00000210857 antibody
    • OTTHUMP00000210858 antibody
    see all

Anti-DAG1 antibody 图像

  • Anti-DAG1 antibody (ab110543) at 1 µg/ml + mouse heart lysate at 10 µg

    Predicted band size : 97 kDa

    gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-DAG1 antibody (ab110543)参考文献

ab110543 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Gating Strategy live cells in fsc vs ssc
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA + 0.1% sodium azide in PBS
Username

Abcam user community

Verified customer

提交于 Apr 30 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2014

Thank you for your inquiry.

I can confirm that we do not know of any publication using the above mentioned antibodies. I would like to reassure you that we do guarantee our product for all species and applications stated on the respective dat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"