概述

  • 产品名称Anti-DAD1抗体
    参阅全部 DAD1 一抗
  • 描述
    兔多克隆抗体to DAD1
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).

  • 阳性对照
    • HepG2 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified by affinity chromatography.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
ICC Use at an assay dependent dilution.

靶标

  • 功能Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
  • 序列相似性Belongs to the DAD/OST2 family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAD 1 antibody
    • DAD-1 antibody
    • dad1 antibody
    • DAD1_HUMAN antibody
    • Defender against cell death 1 antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit DAD1 antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 antibody
    • Oligosaccharyl transferase subunit DAD1 antibody
    • OST 2 antibody
    • OST2 antibody
    see all

Anti-DAD1 antibody 图像

  • Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
    Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
    Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : HepG2 whole cell lysate


    Predicted band size : 12 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.

Anti-DAD1 antibody (ab23836)参考文献

This product has been referenced in:
  • Ding Y  et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . Read more (PubMed: 25301944) »
  • Murooka TT  et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . Read more (PubMed: 19607806) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (skin)
Loading amount 15 µg
Specification skin
Gel Running Conditions Non-reduced Denaturing (15% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2009

Thank you for your enquiry. I contacted the antibody originator regarding ab23836. Unfortunately the amino acid sequence of the immunogen is proprietary. The only information I have is that the sequence was selected from the last half of the protein...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"