Anti-Dact1 / Dapper homolog 1抗体(ab72078)

概述

  • 产品名称Anti-Dact1 / Dapper homolog 1抗体
    参阅全部 Dact1 / Dapper homolog 1 一抗
  • 描述
    兔多克隆抗体to Dact1 / Dapper homolog 1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Xenopus laevis, Chimpanzee, Zebrafish
  • 免疫原

    A portion of amino acids 725-775 of human Dact1/ Dapper homolog 1, (NP_057735).

  • 阳性对照
    • Human, mouse and rat brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately 24 and 110 kDa (predicted molecular weight: 90 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Dact1 / Dapper homolog 1 antibody 图像

    • ab72078 (10µg/ml) staining Dact1 / Dapper homolog 1 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM sodium citrate buffer pH6.0.

    • Lanes 1 - 2 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 1 µg/ml
      Lanes 3 - 4 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 0.5 µg/ml

      Lane 1 : Pre-immune sera
      Lane 2 : Human brain lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate


      Predicted band size : 90 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    Anti-Dact1 / Dapper homolog 1 antibody (ab72078)参考文献

    ab72078 has not yet been referenced specifically in any publications.

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