Anti-Dact1 / Dapper homolog 1抗体(ab51260)

概述

  • 产品名称Anti-Dact1 / Dapper homolog 1抗体
    参阅全部 Dact1 / Dapper homolog 1 一抗
  • 描述
    兔多克隆抗体to Dact1 / Dapper homolog 1
  • 经测试应用适用于: ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fusion protein, corresponding to amino acids 280-516 of Human Dact1/ Dapper homolog 1

性能

应用

Our Abpromise guarantee covers the use of ab51260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/200.
IP 1/100 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
  • 序列相似性Belongs to the dapper family.
  • 结构域The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dact antibody
    • dact1 antibody
    • DACT1_HUMAN antibody
    • Dapper antagonist of beta catenin homolog 1 antibody
    • Dapper antagonist of catenin 1 antibody
    • Dapper antibody
    • Dapper homolog 1 antibody
    • DAPPER1 antibody
    • Dpr antibody
    • Dpr1 antibody
    • Frd antibody
    • Frodo antibody
    • hDPR1 antibody
    • Hepatocellular carcinoma novel gene 3 protein antibody
    • Heptacellular carcinoma novel gene 3 antibody
    • HNG3 antibody
    • MTNG3 antibody
    • Thyex3 antibody
    see all

Anti-Dact1 / Dapper homolog 1 antibody 图像

  • HeLa cells stained with ab51260 Dact1/Dapper homolog 1 antibody (red) at a dilution of 1/200.
  • .... cell extracts immunoprecipitated with ab51260 at 1/200 dilution (lane 2) or a negative control antibody (lane 1). Immunoprecipitates were blotted with ab51260 at 1/1000 dilution.
  • All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab51260) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Molecular weight marker
    Lane 3 : HEK293 transfected with Myc-Dact1 cell lysate


    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • IHC image of ab51260 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51260, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Dact1 / Dapper homolog 1 antibody (ab51260)参考文献

ab51260 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Stem)
Specification Stem
Fixative Paraformaldehyde
Permeabilization Yes - Ethanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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提交于 Mar 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"