概述

  • 产品名称Anti-DACH1抗体[EPR2841Y]
    参阅全部 DACH1 一抗
  • 描述
    兔单克隆抗体[EPR2841Y] to DACH1
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human DACH1

  • 阳性对照
    • MCF-7 and HeLa cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab77234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
Flow Cyt
  • 应用说明Flow Cyt: 1/120.
    IP: 1/40.
    WB: 1/500 - 1/1000.Predicted molecular weight: 79 kDa.

    Is unsuitable for ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
    • 组织特异性Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
    • 序列相似性Belongs to the DACH/dachshund family.
    • 结构域The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DACH antibody
      • Dach1 antibody
      • DACH1_HUMAN antibody
      • dachshund homolog 1 (Drosophila) antibody
      • Dachshund homolog 1 antibody
      • Dachsund homolog 1 antibody
      • FLJ10138 antibody
      • OTTHUMP00000018494 antibody
      see all

    Anti-DACH1 antibody [EPR2841Y] 图像

    • All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 79 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing HeLa cells stained with ab77234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77234, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-DACH1 antibody [EPR2841Y] (ab77234)参考文献

    This product has been referenced in:
    • Lutay N  et al. Bacterial control of host gene expression through RNA polymerase II. J Clin Invest 123:2366-79 (2013). WB ; Human . Read more (PubMed: 23728172) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution - Vector h3300
    Sample Human Tissue sections (adrenal tumour)
    Specification adrenal tumour
    Permeabilization No
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    提交于 Sep 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"