概述

  • 产品名称Anti-DAB2IP抗体
  • 描述
    兔多克隆抗体to DAB2IP
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 850 and 900 of human DAB2IP (CAQ10385.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab87811 was affinity purified using an epitope specific to DAB2IP immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab87811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 相关性DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • AF9Q34 antibody
    • AIP1 antibody
    • ASK interacting protein antibody
    • ASK interacting protein 1 antibody
    • ASK1 interacting protein 1 antibody
    • DAB2 interacting protein antibody
    • DAB2 interaction protein antibody
    • DAB2P antibody
    • DIP1/2 antibody
    • Disabled homolog 2 interacting protein antibody
    • DOC 2/DAB2 interactive protein antibody
    • FLJ39072 antibody
    • KIAA1743 antibody
    • nGAP like protein antibody
    see all

Anti-DAB2IP antibody 图像

  • All lanes : Anti-DAB2IP antibody (ab87811) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 132 kDa
    Observed band size : 132 kDa


    Exposure time : 3 minutes
  • Detection of DAB2IP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87811 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
    Detection: Chemiluminescence with an exposure time of 3 seconds.
  • IHC image of ab87811 staining in normal human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87811, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DAB2IP antibody (ab87811)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"