Anti-Dab1抗体(ab16675)

概述

  • 产品名称Anti-Dab1抗体
    参阅全部 Dab1 一抗
  • 描述
    山羊多克隆抗体to Dab1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 fusion protein.

  • 阳性对照
    • WB: 293 cells transfected with Dab1–HA tag IP: Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate

性能

应用

Our Abpromise guarantee covers the use of ab16675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • 序列相似性Contains 1 PID domain.
  • 结构域The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • 翻译后修饰Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Anti-Dab1 antibody 图像



  • Predicted band size : 67 kDa


    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

Anti-Dab1 antibody (ab16675)参考文献

ab16675 has not yet been referenced specifically in any publications.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Neuroblastoma Cells)
Loading amount 400000 cells
Specification Neuroblastoma Cells
Gel Running Conditions Reduced Denaturing (Gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Mar 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"