Anti-Dab1抗体(ab16675)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Dab1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 fusion protein.

  • 阳性对照
    • WB: 293 cells transfected with Dab1–HA tag. IP: Mouse p14 brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能
    Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • 序列相似性
    Contains 1 PID domain.
  • 结构域
    The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • 翻译后修饰
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Anti-Dab1 antibody 图像



  • Predicted band size : 67 kDa

    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot.

    Lane 1: ab16674 (N terminal).

    Lane 2: ab16675 (C terminal).

    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

Anti-Dab1 antibody (ab16675)参考文献

ab16675 has not yet been referenced specifically in any publications.

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Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Neuroblastoma Cells)
Loading amount
400000 cells
Specification
Neuroblastoma Cells
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Mar 17 2010

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