Anti-Dab1抗体(ab111684)

概述

  • 产品名称Anti-Dab1抗体
    参阅全部 Dab1 一抗
  • 描述
    兔多克隆抗体to Dab1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Pig, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to Human Dab1 aa 1-241. Recombinant fragment corresponding to a region within amino acids 1 and 241 of DAB1 (Uniprot ID#O75553)

  • 阳性对照
    • NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain lysates; HeLa xenograft tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-Dab1 antibody 图像

  • Anti-Dab1 antibody (ab111684) at 1/10000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 60 kDa
  • Anti-Dab1 antibody (ab111684) at 1/3000 dilution + Mouse brain cell lysate at 50 µg

    Predicted band size : 60 kDa
  • ab111684 at 1/500 dilution staining Dab1 in paraffin-embedded HeLa xenograft tissue by Immunohistochemistry.

Anti-Dab1 antibody (ab111684)参考文献

ab111684 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (telencephalons)
Specification telencephalons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 27 2014

Thank you for getting back to us with that update of your progress. I'm pleased to hear that the serum block has worked well for you.

As you have mentioned, the higher background observed in your blots compared to the ones presented on the da...

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Thank you for your recent telephone enquiry, which has been forwarded to me as my colleague Karin is away from the office today. I am sorry to hear you have some concerns regarding the results from ab111684 Anti-Dab1 antibody.

As requested, ...

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Thank you for confirming those details.

As requested, I have generated the testing discount code for you as below:

Discount code: xxxxx

Expiration date: 5th November 2012

Please orderab111684 as normalandquote thi...

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Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

Unfortunately, as discussed over the phone, we do not currently have any antibodies in our catalogue which ...

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Thank you for confirming these details and for your cooperation. We have received no complaints about ab111684 therefore I am concerned about the results you obtained with it; we currently do not have an explanation for the 95 kDa bands, especially as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"