概述

  • 产品名称D4476
  • 描述
    CK1 inhibitor
  • 生物学描述Selective cell-permeable inhibitor of casein kinase 1 (CK1) . (IC50 values are 0.3 (CK1), 0.5 (ALK5), 9.1 (PKd1) and 5.8 μM (p38αMAPK)). Displays selectivity over many other protein kinases.
  • 纯度> 99%

性能

  • 化学名称4-[4-(2,3-Dihydro-1,4-benzodioxin-6-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide
  • 分子量398.41
  • 化学结构Chemical Structure
  • 分子式C23H18N4O3
  • CAS编号301836-43-1
  • PubChem识别号6419753
  • 存放说明Store at +4°C. The product can be stored for up to 12 months.
  • 溶解度概述Soluble in DMSO to 100 mM
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESNC(=O)c1ccc(cc1)c3nc(c2ccccn2)c(n3)c4ccc5OCCOc5c4
  • 来源

    Synthetic

  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab120220 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    D4476 图像

    • ab38511 staining βcatenin (phospho Thr42 + Ser45) in SK-N-SH cells treated with D4476 (ab120220), by ICC/IF. Decrease in expression of βcatenin (phospho Thr42 + Ser45) correlates with increased concentration of D4476, as described in literature.
      The cells were incubated at 37°C for 2h in media containing different concentrations of ab120220 (D4476) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38511 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    D4476 (ab120220)参考文献

    This product has been referenced in:
    • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
    • Rena G  et al. D4476, a cell-permeant inhibitor of CK1, suppresses the site-specific phosphorylation and nuclear exclusion of FOXO1a. EMBO Rep 5:60-5 (2004). Read more (PubMed: 14710188) »

    See all 2 Publications for this product

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