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Abcam's D-Lactate Assay Kit (Colorimetric)Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In this kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (?max = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
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|D-Lactate Assay Buffer||WM||1 x 25ml|
|D-Lactate Enzyme Mix (Lyophilised)||Green||1 x 1ng|
|D-Lactate Standard (100 mM)||Yellow||1 x 100µl|
|D-Lactate Substrate Mix (Lyophilised)||Red||1 vial|
Our Abpromise guarantee covers the use of ab83429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.
Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.
To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.
Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).
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