Fixed tissue cross-reactivity tested with known targets at recommended dilution.
No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against any free D or L amino acid.
The IgG is highly specifc for glucosamine (L/D differences not determined yet).
As most other pentoses are unfixable (lacking primary amino groups), this antibody cannot detect them in fixed tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/2000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.05% using EHPI (Enhanced HPI) with 4% formaldehyde.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
D-glucosamine, or glucosamine-6-phosphate is the biochemical precursor of all nitrogen-containing sugars. It is synthesized from fructose-6-phosphate and glutamine as the first step of the hexosamine biosynthesis pathway. The end-product of this pathway is UDP-N-acetylglucosamine (UDP-GlcNAc), which is then used for making glycosaminoglycans, proteoglycans, and glycolipids.