概述

  • 产品名称Anti-D-Dimer抗体[DD1]
    参阅全部 D-Dimer 一抗
  • 描述
    小鼠单克隆抗体[DD1] to D-Dimer
  • 特异性ab10050 is specific for D-Dimer and high molecular weight fibrin degradation products. No cross-reaction with D-Monomer or D-Dimer treated with mercaptoethanol.
  • 经测试应用适用于: ELISA, WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purity is tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号DD1
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [DD4] to D-Dimer (HRP) (ab10054) and Mouse monoclonal [DD5] to D-Dimer (HRP) (ab10055) and Mouse monoclonal [DD6] to D-Dimer (HRP) (ab24474). Can be used as Capture antibody. Recommended for D-Dimer detection in Human Plasma.

靶标

  • 相关性A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process.
  • 别名
    • D Dimer antibody

Anti-D-Dimer antibody [DD1] 图像

  • D-Dimer, D-monomer and fibrinogen immunodetection by ab10050 by Western blot:

    Lane 1 - D-Dimer
    Lane 2 - D-monomer
    Lane 3 - fibrinogen
    Lane 4 - D-Dimer treated with mercaptoethanol

Anti-D-Dimer antibody [DD1] (ab10050)参考文献

This product has been referenced in:
  • Anderson N  et al. Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring. Int J Colorectal Dis 26:1287-97 (2011). ELISA ; Human . Read more (PubMed: 21698353) »
  • Gulla KC  et al. Activation of mannan-binding lectin-associated serine proteases leads to generation of a fibrin clot. Immunology 129:482-95 (2010). Read more (PubMed: 20002787) »

See all 4 Publications for this product

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The quick way to check if a monoclonal is affected by conjugation is to search for commercially available conjugated versions online. I see that both the DD1 and DD5 clones are available biotinylated, though not ...

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Lamentablemente la secuencia del epítopo no se ha mapeado, y no disponemos de esta información.
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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"