Anti-D Box Binding Protein抗体- BSA and Azide free (ab22824)

概述

  • 产品名称Anti-D Box Binding Protein抗体- BSA and Azide free
    参阅全部 D Box Binding Protein 一抗
  • 描述
    兔多克隆抗体to D Box Binding Protein - BSA and Azide free
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 39-93 of Human D Box Binding Protein

  • 阳性对照
    • HepG2 cell lysate, human liver.

性能

应用

Our Abpromise guarantee covers the use of ab22824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 5 µg/ml. Detects a band of approximately 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
  • 组织特异性Ubiquitously expressed. Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.
  • 序列相似性Belongs to the bZIP family. PAR subfamily.
    Contains 1 bZIP domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Albumin D box-binding protein antibody
    • Albumin D-element-binding protein antibody
    • D site-binding protein antibody
    • DBP antibody
    • DBP_HUMAN antibody
    • Tax-responsive enhancer element-binding protein 302 antibody
    • TAXREB302 antibody
    • TRANSCRIPTION FACTOR DBP antibody
    see all

Anti-D Box Binding Protein antibody - BSA and Azide free 图像

  • Anti-D Box Binding Protein antibody - BSA and Azide free (ab22824) at 1 µg/ml + HepG2 cell lysate

    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • IHC image of DBP at concentrations 4-8ug/ml from paraffin embedded human liver. Arrows indicate hepatocytes with positive labelling.

Anti-D Box Binding Protein antibody - BSA and Azide free (ab22824)参考文献

This product has been referenced in:
  • Meier D  et al. Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes. PLoS One 10:e0137229 (2015). WB . Read more (PubMed: 26361389) »
  • Roussel BD  et al. Age and albumin D site-binding protein control tissue plasminogen activator levels: neurotoxic impact. Brain 132:2219-30 (2009). IHC-P ; Mouse . Read more (PubMed: 19574439) »

See all 2 Publications for this product

Product Wall

Thank you for your patience. The originator of the product have came back to me with a reply and I have attached it below for your reference. You are right. Jérôme Wuarin and Ueli Schibler (Cell, Vol. 63, 1257-1266, December 21, 1990) tested thi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"