CyTRAK Orange™ 50µl (5 mM) (ab109203)
概述
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产品名称
CyTRAK Orange™ 50µl (5 mM) -
经测试应用
适用于: FM, Flow Cyt, ICC/IFmore details -
常规说明
CyTRAK Orange™ is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. It is water soluble and membrane permeant which can be used in live and fixed cells in combination with other common fluorophores, such as GFP-fusions, green-labelled antibodies (FITC or DyLight® 488) and far-red dyes. CyTRAK Orange™ preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations. CyTRAK Orange™ does the work of two fluorophores! Key features of CyTRAK Orange™ include:
- Rapid staining of dsDNA/nuclei of LIVE or fixed cells
- Differential intensity of DNA binding and Cytoplasmic labelling - One fluorophore doing the work of two
- Low photobleaching effect
- Ideal for use with GFP, FITC & DyLight® 488 and 649 labels
- No lyse / no wash protocols
- It is membrane-permeant
Excitation
- 457, 488 & 549 (Exmax 515nm)
- similar profile to Propidium Iodide
- co-excited with FITC/Cy2/eGFP and PE-Cy7
- NOT excited by UV or red lasers (e.g. 633 & 647 nm)
Emission (instrument dependent):
- Emmax 615 nm
- No significant spectral overlap with co-excited green fluors (eGFP, FITC, Cy2)
- Visualize GFP-tagged temporospatial changes in live cells in a single acquisition from a 488 nm excitation
性能
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形式
Liquid -
存放说明
Store at +4°C. Do Not Freeze. Store In the Dark. -
Concentration information loading...
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
1/300 - 1/1000.
For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM |
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ICC/IF |
1/1000. For microscopy counterstain = 5µM
It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet. |
说明 |
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FM
Use at an assay dependent concentration. |
Flow Cyt
1/300 - 1/1000. For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM |
ICC/IF
1/1000. For microscopy counterstain = 5µM It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109203 尚未被引用在任何文献中。