Cytosol/Particulate Rapid Separation试剂盒(ab65398)

概述

  • 产品名称
    Cytosol/Particulate Rapid Separation试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型
    Direct
  • 检测时间
    1h 00m
  • 产品概述

    Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 标识符 50 tests
    Cell Suspension Buffer Red 1 x 2ml
    Cytosol Releasing Buffer Green 1 x 2ml
    Oil Layer WM 1 x 25ml
    Particulate Layer Blue 1 x 2ml
  • 研究领域
  • 相关性
    Cytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.

应用

Our Abpromise guarantee covers the use of ab65398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

文献

ab65398 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Particulate fractions are the unsoluble fractions of lysaed cells e.g. plasma membrane. You are correct!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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