CytoPainter Mitochondrial Staining试剂盒- Orange Fluorescence (ab112144)

概述

  • 产品名称CytoPainter Mitochondrial Staining试剂盒- Orange Fluorescence
    参阅全部 Mitochondria 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based
  • 产品概述

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112144 is designed to label mitochondria in live cells with orange fluorescence at Ex/Em = 545/575nm (TRITC filter-compatible). This kits uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. ab112144 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry.

  • 说明

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • 经测试应用适用于: FM, ICC/IFmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112144 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter Mitochondrial Staining Kit - Orange Fluorescence 图像

    • Image of U2OS cells stained with ab112144 in a black 96-well plate

    实验方案

    CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144)参考文献

    ab112144 has not yet been referenced specifically in any publications.

    Product Wall

    J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
    1) peuvent être utilisés sur des cellules vivantes,
    2) ne stoppent pas la division cellulaire,
    3) Ne sont à priori pas to...

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    Merci de nous avoir contactés.

    Le Draq5 se lie à l'ADN et empêche donc malheureusement la division cellulaire.

    Un autre produit me semble intéressant: CytoPainter Mitochondrial Staining Kit - Orange Fluoresc...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"