CytoPainter Mitochondrial Staining试剂盒- Green Fluorescence (ab112143)

概述

  • 产品名称CytoPainter Mitochondrial Staining试剂盒- Green Fluorescence
    参阅全部 Mitochondria 试剂盒
  • 测试
    1 x 500 test
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based
  • 产品概述

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    CytoPainter Mitochondrial Staining Kit - Green Fluorescence (ab112143) is designed to label mitochondria in live cells with green fluorescence at Ex/Em = 490/520 nm. The kit uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.

     

  • 说明

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • 经测试应用适用于: FM, ICC/IFmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112143 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter Mitochondrial Staining Kit - Green Fluorescence 图像

    • Image of U2OS cells stained with ab112143 CytoPainter Mitochondrial Staining Kit - Green Fluorescence in a black 96-well plate.
    • HeLa cells were incubated for 60 minutes at 37C with ab112143. The staining solution was replaced with 4%PFA for 10 minutes before a final wash in PBS prior to mounting slides.

      Images obtained with an Olympus BX61 microscope using a FITC filter (495/519 nm) - 1750ms exposure.

    实验方案

    CytoPainter Mitochondrial Staining Kit - Green Fluorescence (ab112143)参考文献

    ab112143 has not yet been referenced specifically in any publications.

    Product Wall

    1) Most likely not, it is for live cell mitochondria staining.
    2) We don't have experiences on Drosophila mitochondria, it should work theoretically.

    Sorry for the delay in getting back to you.

    As your customer has commented, the live cell mitochondria stain, will also stain the mitochondria of dead cells. The product ab112145 uses a red fluorescent stain for this and will decrease the fluo...

    Read More

    Thank you for your call today and for your enquiry.

    As we discussed, I would recommend either ab14744 or ab15895 for immunostaining of mitochondria in mouse cells-

    http://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-C...

    Read More

    Thank you for contacting Abcam.

    After you have labeled the live cells with the Cytopainter kit, you can then fix the cells using formaldehyde.

    Please let me know if there is anything else I can help you with.

    Thank you for your enquiry. The mitochondria dye for ab112143 is for live cells, and can't used after the cells were fixed.  However, our ab112143 kit is fixable (meaning that it labels the live cells, and then the cells can be fixed by formaldehyde...

    Read More

    Thank you for your enquiry. I am sorry to confirm this kit has not been tested on live cells. We would suggest that incubation with ab112143 for a few hours should be ok, but not for a few days. However, regrettably we are not able to guarantee this as...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"