CytoPainter Lysosomal Staining试剂盒- Green Fluorescence (ab112136)

概述

  • 产品名称CytoPainter Lysosomal Staining试剂盒- Green Fluorescence
    参阅全部 Lysosome 试剂盒
  • 测试
    1 x 500 test
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based
  • 产品概述

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112136 is designed to label lysosomes in live cells with green fluorescence at Ex/Em = 490/525 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

  • 经测试应用适用于: FMmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.

    CytoPainter Lysosomal Staining Kit - Green Fluorescence 图像

    • ab112136 staining U2OS cells by fluorescence microscopy (in a black 96 well plate).

    实验方案

    CytoPainter Lysosomal Staining Kit - Green Fluorescence (ab112136)参考文献

    This product has been referenced in:
    • Aasen SN  et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). Read more (PubMed: 26370983) »

    See 1 Publication for this product

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    They are mostly in lysosomes, but also may go to acidic organelles.


    The green fluorescence of the ab112136 lysosome stain does not persist after PFA fixation, but the fluorescence of CytoPainter lysosome stains that contain these other fluorochromes does:

    Orange - ab138895

    Red - ab112137
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