CytoPainter Lysosomal Staining试剂盒- Blue Fluorescence (ab112135)

概述

  • 产品名称CytoPainter Lysosomal Staining试剂盒- Blue Fluorescence
    参阅全部 Lysosome 试剂盒
  • 测试
    1 x 500 test
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based
  • 产品概述

    CytoPainter Lysosomal Staining Kit - Blue Fluorescence (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. Ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.

    Ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. Ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

  • 说明

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

  • 经测试应用适用于: FM, ICC/IFmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter Lysosomal Staining Kit - Blue Fluorescence 图像

    • ab112135 staining U2OS cells by Immunofluorescence (in a black 96 well plate).

    实验方案

    CytoPainter Lysosomal Staining Kit - Blue Fluorescence (ab112135)参考文献

    ab112135 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your inquiry. I am sorry to hear you have been experiencing problems with the CytoPainter Lysosomal Staining Kit. Due to the wide emission spectrum property of the CytoPainter dye it is normal to also have some green fluo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"