Lysosomal Staining试剂盒- Blue Fluorescence - Cytopainter (ab112135)
概述
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产品名称
Lysosomal Staining试剂盒- Blue Fluorescence - Cytopainter
参阅全部 Lysosomes 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based -
产品概述
Lysosomal Staining Kit - Blue Fluorescence | Cytopainter (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. Ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.
Ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. Ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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说明
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
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平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests Live Cell Staining Buffer 1 x 50ml LysoBlue Indicator 1 x 100µl -
研究领域
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别名
- lysosome marker
- lysotracker
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab112135 被引用在 4 文献中.
- LeBlond ND et al. Foam Cell Induction Activates AMPK But Uncouples Its Regulation of Autophagy and Lysosomal Homeostasis. Int J Mol Sci 21:N/A (2020). PubMed: 33261140
- Purdie L et al. Alkyl-Modified Oligonucleotides as Intercalating Vehicles for Doxorubicin Uptake via Albumin Binding. Mol Pharm 15:437-446 (2018). PubMed: 29265823
- Liu F et al. Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo. Int J Nanomedicine 12:5135-5148 (2017). PubMed: 28790821
- Purdie L et al. Influence of Polymer Size on Uptake and Cytotoxicity of Doxorubicin-Loaded DNA-PEG Conjugates. Bioconjug Chem 27:1244-52 (2016). PubMed: 27074730