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CytoPainter F-actin Staining Kit (ab112126) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.
ab112126 should be stored dessicated.
|Labeling Buffer||1 x 50ml|
|Orange Fluorescent Phalloidin Conjugate||1 x 50µl|
Our Abpromise guarantee covers the use of ab112126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab112126 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"