Anti-Cytokeratin 8+18抗体[K8.8 + DC10] (ab80571)

概述

  • 产品名称Anti-Cytokeratin 8+18抗体[K8.8 + DC10]
    参阅全部 Cytokeratin 8+18 一抗
  • 描述
    小鼠单克隆抗体[K8.8 + DC10] to Cytokeratin 8+18
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Semi-purified Human cytokeratin preparation.

  • 阳性对照
    • Skin or breast carcinoma.
  • 常规说明

    Clone K8.8+ DC10 Ig Isotype / Light Chain: IgG1 / kappa + IgG1.

    ab80571 recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明ab80571 recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative.
  • 克隆单克隆
  • 克隆编号K8.8 + DC10
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use for 30 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

靶标

  • 相关性Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • CARD2 antibody
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK18 antibody
    • CK8 antibody
    • CYK18 antibody
    • CYK8 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-8 antibody
    • K18 antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 18 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • KRT18 antibody
    • KRT8 antibody
    • type II cytoskeletal 8 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8+18 antibody [K8.8 + DC10] 图像

  • Formalin-fixed, paraffin-embedded human breast cancer stained with ab80571 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.

Anti-Cytokeratin 8+18 antibody [K8.8 + DC10] (ab80571)参考文献

This product has been referenced in:
  • van der Loos CM  et al. Accurate quantitation of Ki67-positive proliferating hepatocytes in rabbit liver by a multicolor immunohistochemical (IHC) approach analyzed with automated tissue and cell segmentation software. J Histochem Cytochem 61:11-8 (2013). Read more (PubMed: 22941418) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"