RabMAb

Anti-Cytokeratin 8 (phospho S73)抗体[E431-2] (ab32579)

概述

  • 产品名称Anti-Cytokeratin 8 (phospho S73)抗体[E431-2]
    参阅全部 Cytokeratin 8 一抗
  • 描述
    兔单克隆抗体[E431-2] to Cytokeratin 8 (phospho S73)
  • 特异性The antibody only detects Keratin 8 phosphorylated on Serine 73. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Cytokeratin 8. Isolated from cells pretreated with okadaic acid or pervanadate to promote Serine hyperphosphorylation,

  • 阳性对照
    • WB: A431 cell lysate ICC/IF: A431 cells IHC: liver carcinoma
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 19409407
WB 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关Cirrhosis
    • 序列相似性Belongs to the intermediate filament family.
    • 翻译后修饰Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] 图像

    • Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (ab32579) diluted 1/250.
    • IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 (ab32579) diluted 1/500.
    • Ab32579 staining cells from a human pancreatic cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

      See Abreview

    • Immunohistochemical analysis of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with ab32579.

      Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

      See Abreview

    Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] (ab32579)参考文献

    This product has been referenced in:
    • Kuga T  et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci 126:4721-31 (2013). WB ; Human . Read more (PubMed: 23902688) »
    • Kongara S  et al. Autophagy regulates keratin 8 homeostasis in mammary epithelial cells and in breast tumors. Mol Cancer Res 8:873-84 (2010). WB ; Mouse . Read more (PubMed: 20530580) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human cancer cell line A549)
    Loading amount 25 µg
    Specification human cancer cell line A549
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Username

    Abcam user community

    Verified customer

    提交于 Sep 28 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (lung adenoma)
    Specification lung adenoma
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 25 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (lung adenoma)
    Specification lung adenoma
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (lung cancer primary tumor)
    Loading amount 25 µg
    Specification lung cancer primary tumor
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount 20 µg
    Specification pancreatic cancer cell line
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Pancreatic cancer cell line)
    Specification Pancreatic cancer cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Dr. Hannah Whiteman

    Verified customer

    提交于 Jun 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"