Anti-Cytokeratin 8抗体[TS1] (Biotin) (ab79471)

概述

  • 产品名称Anti-Cytokeratin 8抗体[TS1] (Biotin)
    参阅全部 Cytokeratin 8 一抗
  • 描述
    小鼠单克隆抗体[TS1] to Cytokeratin 8 (Biotin)
  • 偶联物Biotin
  • 特异性Highly specific to Cytokeratin 8; shows no cross-reactivity with other keratins. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Keratin preparation from a Human carcinoma

  • 表位aa 343-357
  • 阳性对照
    • IP: MCF-7 or A431 cells. IHC-P: Human skin; Human colon, lung or breast carcinoma tissue.
  • 常规说明Stable for 24 months when stored at 4°C.

性能

应用

Our Abpromise guarantee covers the use of ab79471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
IHC-P
  • 应用说明IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Use protein G.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关Cirrhosis
    • 序列相似性Belongs to the intermediate filament family.
    • 翻译后修饰Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [TS1] (Biotin) (ab79471)参考文献

    This product has been referenced in:
    • Sundström BE  et al. Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J Histochem Cytochem 37:1845-54 (1989). IHC-P ; Human . Read more (PubMed: 2479674) »

    See 1 Publication for this product

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