概述

  • 产品名称
    Anti-Cytokeratin 8抗体[TS1]
    参阅全部 Cytokeratin 8 一抗
  • 描述
    小鼠单克隆抗体[TS1] to Cytokeratin 8
  • 特异性
    ab49778 recognises cytokeratin 8 intermediate filament.
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified tumour-derived cytokeratins.

  • 阳性对照
    • IHC-P: Human lung tissue. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab49778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100 - 1/200.

Acetone fixation recommended.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性
      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关
      Cirrhosis
    • 序列相似性
      Belongs to the intermediate filament family.
    • 翻译后修饰
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位
      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    图片

    • ICC/IF image of ab49778 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49778, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Immunostaining of human lung tissue with ab49778.

    文献

    This product has been referenced in:
    • Lehman HL  et al. p120-catenin down-regulation and epidermal growth factor receptor overexpression results in a transformed epithelium that mimics esophageal squamous cell carcinoma. Am J Pathol 185:240-51 (2015). Read more (PubMed: 25529795) »
    • Holm P  et al. Functional mapping and single chain construction of the anti-cytokeratin 8 monoclonal antibody TS1. Mol Immunol 44:1075-84 (2007). Read more (PubMed: 16989900) »

    See all 5 Publications for this product

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