Anti-Cytokeratin 8抗体[C-43] (ab2530)

概述

  • 产品名称
    Anti-Cytokeratin 8抗体[C-43]
    参阅全部 Cytokeratin 8 一抗
  • 描述
    小鼠单克隆抗体[C-43] to Cytokeratin 8
  • 特异性
    This antibody recognises the 52.5kDa Cytokeratin 8.
  • 经测试应用
    适用于: ICC, WB, IHC-P, IP, Flow Cytmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Cow, Human, Pig
    不与反应: Mouse, Rat, Chicken, Xenopus laevis
  • 免疫原

    Tissue/ cell preparation (Human). Cytoskeleton preparation from HeLa cells.

  • 阳性对照
    • IHC-P: Human lung FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab2530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 52.5 kDa.
IHC-P Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

靶标

  • 功能
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 组织特异性
    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • 疾病相关
    Cirrhosis
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 细胞定位
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

图片

  • Overlay histogram showing MCF7 cells stained with ab2530 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2530, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
  • IHC image of ab2530 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2530, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab2530 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2530, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Canciello A  et al. Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties. Sci Rep 7:3761 (2017). IHC-P ; Sheep . Read more (PubMed: 28630448) »
  • Elakoum R  et al. CARM1 and PRMT1 are dysregulated in lung cancer without hierarchical features. Biochimie 97:210-8 (2014). WB ; Human . Read more (PubMed: 24211191) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your phone call. Regarding ab2530, ab2531, and ab8477, the epitopes have not been determined. Once I have heard back from the originator of ab9023 I will let you know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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