The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
1/50 - 1/100.
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Belongs to the intermediate filament family.
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue section labeling Cytokeratin 8 with ab53708. The picture on the right is blocked with the synthesized peptide.
Western blot - Cytokeratin 8 antibody (ab53708)
All lanes : Anti-Cytokeratin 8 antibody (ab53708) at 1/500 dilution
Lane 1 : SKOV3 cell extract Lane 2 : SKOV3 cell extract with immunising peptide
Predicted band size : 54 kDa Observed band size : 54 kDa