Anti-Cytokeratin 8抗体(ab52949)

概述

  • 产品名称Anti-Cytokeratin 8抗体
    参阅全部 Cytokeratin 8 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 8
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 8

  • 阳性对照
    • HeLa cell lysate, human gastric adenocarcinoma and HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关Cirrhosis
    • 序列相似性Belongs to the intermediate filament family.
    • 翻译后修饰Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody 图像

    • Anti-Cytokeratin 8 antibody (ab52949) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in human gastric adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in HeLa cells by Immunofluorescence.

    Anti-Cytokeratin 8 antibody (ab52949)参考文献

    ab52949 has not yet been referenced specifically in any publications.

    Product Wall

    Les dilutions recommandées pour ab52949 sont :
    Western Blot : 1/500
    IHC-P et ICC/IF : 1/100 - 1/250
    IP : 1/60

    Pour l'anticorps ab51152, la concentration est de 1mg/ml. L'immunogèneayant servi à produire cet...

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    Je vous confirme que l'anticorpsab52949 est dirigé contre la partie N-terminale de la cytokeratine 8 humaine.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"