Anti-Cytokeratin 8抗体[35bH11] (ab758)

概述

  • 产品名称Anti-Cytokeratin 8抗体[35bH11]
    参阅全部 Cytokeratin 8 一抗
  • 描述
    小鼠单克隆抗体[35bH11] to Cytokeratin 8
  • 特异性This antibody recognizes a keratin polypeptide of 54 kDa. It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinoma of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts stain positive with this antibody.
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B

性能

应用

Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
  • 应用说明IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
  • 靶标

    • 功能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关Cirrhosis
    • 序列相似性Belongs to the intermediate filament family.
    • 翻译后修饰Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [35bH11] 图像

    • ab758 - immunohistochemistry

      Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

    • ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

    Anti-Cytokeratin 8 antibody [35bH11] (ab758)参考文献

    This product has been referenced in:
    • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »

    See 1 Publication for this product

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    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"