概述

  • 产品名称Anti-Cytokeratin 7+17抗体[C-46]
  • 描述
    小鼠单克隆抗体[C-46] to Cytokeratin 7+17
  • 特异性Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)
  • 经测试应用适用于: ICC, WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Sheep, Cow, Dog, Human, Pig
    不与反应: Mouse, Rat, Rabbit
  • 免疫原

    Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).

  • 常规说明Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from culture supernatant using protein A-affinity chromatography.
  • 克隆单克隆
  • 克隆编号C-46
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
WB
IHC-P
IP
  • 应用说明ICC: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    IP: Use at an assay dependant dilution.
    WB: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • CK17 antibody
      • CK7 antibody
      • Cytokeratin 17 antibody
      • Cytokeratin 7 antibody
      • K17 antibody
      • K7 antibody
      • Keratin 17 antibody
      • Keratin 7 antibody
      • KRT17 antibody
      • KRT7 antibody
      see all

    Anti-Cytokeratin 7+17 antibody [C-46] 图像

    • Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate

      Observed band size : 49,51 kDa (why is the actual band size different from the predicted?)
    • Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    Anti-Cytokeratin 7+17 antibody [C-46] (ab670)参考文献

    This product has been referenced in:
    • Sun Y  et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). Read more (PubMed: 19524636) »
    • Vojtesek B  et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). Read more (PubMed: 1695327) »

    See all 2 Publications for this product

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