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Cytokeratins from the human bladder carcinoma cell line T24.
Our Abpromise guarantee covers the use of ab9021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/100 - 1/500.|
|ICC||1/100 - 1/500.|
|WB||1/100 - 1/1000.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19233130|
ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
ab9021 staining Cytokeratin 7 in mouse liver, heterogeneous primary cell culture by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 3% Mouse Ig Blocking reagent (component of a commercially available mouse on mouse MOM kit) at 200C for 30 minutes. Samples were incubated with primary antibody (1/100: in dilution buffer from MOM kit) for 1 hour at 200C. An Alexa Fluor ® 568-conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/250 dilution.
Image courtesy of an anonymous Abreview.Blocked with 3% BSA for 2 hours.
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