Anti-Cytokeratin 7抗体[RCK105] (ab9021)

概述

  • 产品名称
    Anti-Cytokeratin 7抗体[RCK105]
    参阅全部 Cytokeratin 7 一抗
  • 描述
    小鼠单克隆抗体[RCK105] to Cytokeratin 7
  • 特异性
    This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, ICC, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Goat, Hamster, Cat, Dog, Human, Pig, Zebrafish
  • 免疫原

    Cytokeratins from the human bladder carcinoma cell line T24.

  • 阳性对照
    • mouse ovary tissue, breast tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    RCK105
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/100 - 1/500.
ICC 1/100 - 1/500.
WB 1/100 - 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 19233130
IHC-P 1/1000.

靶标

  • 功能
    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • 组织特异性
    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CK 7 antibody
    • CK-7 antibody
    • CK7 antibody
    • Cytokeratin 7 antibody
    • Cytokeratin-7 antibody
    • D15Wsu77e antibody
    • K2C7 antibody
    • K2C7_HUMAN antibody
    • K7 antibody
    • Keratin 7 antibody
    • Keratin 7, type II antibody
    • Keratin type II cytoskeletal 7 antibody
    • Keratin, 55K type II cytoskeletal antibody
    • Keratin, simple epithelial antibody
    • Keratin, simple epithelial type I, K7 antibody
    • Keratin, type II cytoskeletal 7 antibody
    • Keratin-7 antibody
    • Krt2-7 antibody
    • KRT7 antibody
    • MGC11625 antibody
    • MGC129731 antibody
    • MGC3625 antibody
    • Sarcolectin antibody
    • SCL antibody
    • Type II mesothelial keratin K7 antibody
    • Type-II keratin Kb7 antibody
    see all

Anti-Cytokeratin 7 antibody [RCK105] 图像

  • Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
  • ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.

  • ab9021 staining breast epithelial cell line. The cells are fixated in Methanol. These pictures are part of the review submitted by Rene Villadsen.
  • Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection. Review by Todd Gocken submitted 21 July 2004

  • ab9021 staining Cytokeratin 7 in mouse liver, heterogeneous primary cell culture by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 3%  Mouse Ig Blocking reagent (component of a commercially available mouse on mouse MOM kit) at 200C for 30 minutes. Samples were incubated with primary antibody (1/100: in dilution buffer from MOM kit) for 1 hour at 200C. An Alexa Fluor ® 568-conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/250 dilution.

    See Abreview

  • All lanes : Anti-Cytokeratin 7 antibody [RCK105] (ab9021) at 1/200 dilution

    Lane 1 : Whole tissue lysate prepared from human pancreatic tumor tissue.
    Lane 2 : Whole tissue lysate prepared from human pancreatic tumor tissue.

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-mouse HRP at 1/10000 dilution
    Developed using the ECL technique

    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    Blocked with 3% BSA for 2 hours.

    See Abreview

  • Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes

Anti-Cytokeratin 7 antibody [RCK105] (ab9021)参考文献

This product has been referenced in:
  • Kim JY  et al. Apamin suppresses biliary fibrosis and activation of hepatic stellate cells. Int J Mol Med 39:1188-1194 (2017). IHC-P ; Mouse . Read more (PubMed: 28405682) »
  • Ambade A  et al. Alcoholic hepatitis accelerates early hepatobiliary cancer by increasing stemness and miR-122-mediated HIF-1a activation. Sci Rep 6:21340 (2016). IHC-P . Read more (PubMed: 26888602) »

See all 24 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (thyroid follicular tumor, follicular cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
thyroid follicular tumor, follicular cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviductal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Specification
oviductal tissues
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 16 2016

Thank you for contacting us.

You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative c...

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I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibod...

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Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human pancreatic tumor)
Loading amount
20 µg
Specification
Human pancreatic tumor
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Nov 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Liver)
Specification
Liver
Fixative
95% ETOH
Permeabilization
No
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Dec 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Squamous cell carcinoma / cell line SCC4)
Specification
Squamous cell carcinoma / cell line SCC4
Fixative
Ethanol:Aceton 1:1
Permeabilization
No
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Apr 20 2010

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