Anti-Cytokeratin 7抗体[RCK105] - Cytoskeleton Marker (ab9021)
Key features and details
- Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 7抗体[RCK105] - Cytoskeleton Marker
参阅全部 Cytokeratin 7 一抗 -
描述
小鼠单克隆抗体[RCK105] to Cytokeratin 7 - Cytoskeleton Marker -
宿主
Mouse -
特异性
This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. -
经测试应用
适用于: IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Human, Pig
预测可用于: Rat, Goat, Hamster, Cat, Dog, Zebrafish -
免疫原
Full length native protein (purified) corresponding to Human Cytokeratin 7. BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
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阳性对照
- IHC-P: Human liver tissue, mouse ovary and bladder tissue IHC-Fr: Human liver, urinary bladder tissue, Pig liver and skin tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
RCK105 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
靶标
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功能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3855 Human
- Entrez Gene: 300242 Rat
- Omim: 148059 Human
- SwissProt: P08729 Human
- SwissProt: Q6IG12 Rat
- Unigene: 411501 Human
- Unigene: 670221 Human
- Unigene: 7913 Rat
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别名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
图片
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Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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ab9021 staining normal pig skin tissue section (frozen), staining epithelial cells using ab9021 at 1/500.
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Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre
Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).
Cytokeratin 7 was detected using ab9021.
(After Figure 3 of Yang et al)
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ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
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Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.
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Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
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Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes
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Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (93)
ab9021 被引用在 93 文献中.
- Gross A et al. Desmoplakin Maintains Transcellular Keratin Scaffolding and Protects From Intestinal Injury. Cell Mol Gastroenterol Hepatol 13:1181-1200 (2022). PubMed: 34929421
- Zhou WJ et al. Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2+ macrophage differentiation. Sci Adv 8:eabj2488 (2022). PubMed: 35196096
- Wang LJ et al. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation. Nat Commun 13:1626 (2022). PubMed: 35338152
- Mauduit O et al. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39:110663 (2022). PubMed: 35417692
- Shibata O et al. Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method. Mol Ther Nucleic Acids 28:342-352 (2022). PubMed: 35474735