重组
RabMAb

Anti-Cytokeratin 7抗体[EPR1619Y] - Cytoskeleton Marker (ab68459)

概述

  • 产品名称
    Anti-Cytokeratin 7抗体[EPR1619Y] - Cytoskeleton Marker
    参阅全部 Cytokeratin 7 一抗
  • 描述
    兔单克隆抗体[EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human Cytokeratin 7 aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa, SK-OV-3 and T47D cell lysates. IHC-P: Human ovarian carcinoma and human bladder carcinoma tissues. ICC/IF: HeLa and TD47 cells. Flow Cyt: HeLa cells. IP: HeLa cell lysate.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab68459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/20 - 1/30.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Flow Cyt 1/20 - 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

靶标

  • 功能
    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • 组织特异性
    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CK 7 antibody
    • CK-7 antibody
    • CK7 antibody
    • Cytokeratin 7 antibody
    • Cytokeratin-7 antibody
    • D15Wsu77e antibody
    • K2C7 antibody
    • K2C7_HUMAN antibody
    • K7 antibody
    • Keratin 7 antibody
    • Keratin 7, type II antibody
    • Keratin type II cytoskeletal 7 antibody
    • Keratin, 55K type II cytoskeletal antibody
    • Keratin, simple epithelial antibody
    • Keratin, simple epithelial type I, K7 antibody
    • Keratin, type II cytoskeletal 7 antibody
    • Keratin-7 antibody
    • Krt2-7 antibody
    • KRT7 antibody
    • MGC11625 antibody
    • MGC129731 antibody
    • MGC3625 antibody
    • Sarcolectin antibody
    • SCL antibody
    • Type II mesothelial keratin K7 antibody
    • Type-II keratin Kb7 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

  • Immunocytochemistry/Immunofluorescence analysis of T47D cells labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • All lanes : Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) at 1/10000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SK-OV-3 whole cell lysate
    Lane 3 : T47D whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry analysis of HeLa cells labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/20 (red). Cells were fixed with 80% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab68459 (purified) at 1/20 immunoprecipitating Cytokeratin 7 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab68459 + HeLa whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68459 in HeLa whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) at 1/10000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : T47D cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Cytokerain 7 with unpurified ab68459 at a dilution of 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder transitional carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

  • ab68459 showing negative staining in human sarcoma tissue.

  • ab68459 showing negative staining in human colonic adenocarcinoma tissue.

  • Overlay histogram showing HeLa cells stained with unpurified ab68459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab68459, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:
  • Francavilla C  et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017). ICC/IF ; Human . Read more (PubMed: 28355574) »
  • Weisblum Y  et al. Zika Virus Infects Early- and Midgestation Human Maternal Decidual Tissues, Inducing Distinct Innate Tissue Responses in the Maternal-Fetal Interface. J Virol 91:N/A (2017). IHC-P . Read more (PubMed: 27974560) »

See all 7 Publications for this product

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