重组Anti-Cytokeratin 7抗体[EP1620Y] (ab68460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1620Y] to Cytokeratin 7
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 7抗体[EP1620Y]
参阅全部 Cytokeratin 7 一抗 -
描述
兔单克隆抗体[EP1620Y] to Cytokeratin 7 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Cytokeratin 7 aa 450-550 (C terminal).
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阳性对照
- WB: HeLa, T-47D, SK-BR-3, A549 and placenta cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1620Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IP |
1/20.
For unpurified use at 1/50. |
说明 |
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WB
1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IP
1/20. For unpurified use at 1/50. |
靶标
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功能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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别名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
图片
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All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/10000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : KRT7 knockout A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 40-55 kDa why is the actual band size different from the predicted?Western blot: Anti-KRT7 antibody [EP1620Y] (ab68460) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab68460 was shown to bind specifically to KRT7. A band was observed at 40-55 kDa in wild-type A549 cell lysates with no signal observed at this size in KRT7 knockout cell line. To generate this image, wild-type and KRT7 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab68460 & T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg/ml
Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 4 : Human placenta tissue lysate at 20 mg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDaBlocking and diluting buffer : 5% NFDM/TBST
~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform.[PMID:16265353]
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Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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