Anti-Cytokeratin 6抗体(ab111540)


  • 产品名称
    Anti-Cytokeratin 6抗体
    参阅全部 Cytokeratin 6 一抗
  • 描述
    兔多克隆抗体to Cytokeratin 6
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cytokeratin 6.

  • 阳性对照
    • This antibody gave a positive signal in A431 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenocarcinoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A431



Our Abpromise guarantee covers the use of ab111540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


  • 组织特异性
    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
  • 序列相似性
    Belongs to the intermediate filament family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CK 6A antibody
    • CK 6B antibody
    • CK 6C antibody
    • CK 6D antibody
    • CK 6E antibody
    • CK-6C antibody
    • CK-6E antibody
    • Cytokeratin 6a antibody
    • Cytokeratin 6B antibody
    • Cytokeratin 6C antibody
    • Cytokeratin 6D antibody
    • Cytokeratin 6E antibody
    • Cytokeratin-6C antibody
    • Cytokeratin-6E antibody
    • K2C6C_HUMAN antibody
    • K6a keratin antibody
    • K6b keratin antibody
    • K6C antibody
    • K6c keratin antibody
    • K6d keratin antibody
    • K6e keratin antibody
    • Keratin antibody
    • Keratin K6h antibody
    • Keratin type II cytoskeletal 6A antibody
    • Keratin type II cytoskeletal 6B antibody
    • Keratin type II cytoskeletal 6C antibody
    • Keratin type II cytoskeletal 6D antibody
    • Keratin type II cytoskeletal 6E antibody
    • Keratin-6C antibody
    • KRT6A antibody
    • KRT6B antibody
    • KRT6C antibody
    • KRT6D antibody
    • KRT6E antibody
    • type II cytoskeletal 6C antibody
    • Type-II keratin Kb12 antibody
    see all


  • Anti-Cytokeratin 6 antibody (ab111540) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 12 kDa,53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC image of Cytokeratin 6 staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab111540, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab111540 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111540 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab111540 has not yet been referenced specifically in any publications.


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