Anti-Cytokeratin 5 + 18抗体[C-50] (ab2535)


  • 产品名称Anti-Cytokeratin 5 + 18抗体[C-50]
  • 描述
    小鼠单克隆抗体[C-50] to Cytokeratin 5 + 18
  • 特异性This antibody recognises the 58 kDa Cytokeratin 5 and the 45 kDa Cytokeratin 18.
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Human, Pig
    不与反应: Chicken, Xenopus laevis
  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 5 + 18.

  • 常规说明This antibody is strongly positive on simple and stratified epithelia.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein A-affinity chromatography.
  • 克隆单克隆
  • 克隆编号C-50
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab2535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


Anti-Cytokeratin 5 + 18 antibody [C-50] 图像

  • ICC/IF image of ab2535 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2535, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A431 cells stained with ab2535 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2535, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin 5 + 18 antibody [C-50] (ab2535)参考文献

This product has been referenced in:
  • Bártek J  et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). Read more (PubMed: 1716305) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Dog Lens Epithelial (DLE) cells (primary culture))
Specification Dog Lens Epithelial (DLE) cells (primary culture)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

Miss. Amanda Agler

Verified customer

提交于 Jan 31 2006