概述

  • 产品名称Anti-Cytokeratin 5 + 14抗体[LH8]
    参阅全部 Cytokeratin 5 + 14 一抗
  • 描述
    小鼠单克隆抗体[LH8] to Cytokeratin 5 + 14
  • 特异性Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The 50,000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号LH8
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgM
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 24705787
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
  • 细胞定位Cytoplasmic; Basal layer
  • 数据库链接
  • 别名
    • CK 14 antibody
    • CK 5 antibody
    • DDD antibody
    • k14 antibody
    • k5 antibody
    • Keratin 14 antibody
    • Keratin 5 antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin type II cytoskeletal 5 antibody
    • krt14 antibody
    • krt5 antibody
    see all

Anti-Cytokeratin 5 + 14 antibody [LH8] (ab20203)参考文献

This product has been referenced in:
  • Moser B  et al. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS One 9:e94118 (2014). IHC-P ; Human . Read more (PubMed: 24705787) »
  • Prince ME  et al. Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci U S A 104:973-8 (2007). IHC-Fr ; Human . Read more (PubMed: 17210912) »

See all 2 Publications for this product

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