Anti-Cytokeratin 5 + 14抗体[16.4] (ab16570)
Key features and details
- Mouse monoclonal [16.4] to Cytokeratin 5 + 14
- Suitable for: IHC-Fr, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cytokeratin 5 + 14抗体[16.4]
参阅全部 Cytokeratin 5 + 14 一抗 -
描述
小鼠单克隆抗体[16.4] to Cytokeratin 5 + 14 -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
This product was produced with the following immunogens:
Tissue, cells or virus corresponding to Cat Cytokeratin 5 + 14. cytokeratin enriched extract of feline tongue epithelium
Tissue, cells or virus corresponding to Mouse Cytokeratin 5 + 14. mouse myeloma cells -
常规说明
Cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
16.4 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. -
细胞定位
Cytoplasmic; Basal layer -
数据库链接
- Entrez Gene: 3852 Human
- Entrez Gene: 3861 Human
- Omim: 148040 Human
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: P13647 Human
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别名
- CK 14 antibody
- CK 5 antibody
- DDD antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab16570 被引用在 3 文献中.
- Arthur GK et al. KCa3.1 K+ Channel Expression and Function in Human Bronchial Epithelial Cells. PLoS One 10:e0145259 (2015). PubMed: 26689552
- Woodman LB et al. Synthetic response of stimulated respiratory epithelium: modulation by prednisolone and iKK2 inhibition. Chest 143:1656-66 (2013). ICC/IF ; Human . PubMed: 23238614
- Stenzinger A et al. Epidermal growth factor-, transforming growth factor-beta-, retinoic acid- and 1,25-dihydroxyvitamin D3-regulated expression of the novel protein PTPIP51 in keratinocytes. Cells Tissues Organs 184:76-87 (2006). PubMed: 17361080