The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (from PubMed: 17361080)
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.