概述

  • 产品名称Anti-Cytokeratin 4抗体[EPR1598]
    参阅全部 Cytokeratin 4 一抗
  • 描述
    兔单克隆抗体[EPR1598] to Cytokeratin 4
  • 特异性The immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues within Human Cytokeratin 4.

  • 阳性对照
    • WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 57 kDa.

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
    • 疾病相关Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • 序列相似性Belongs to the intermediate filament family.
    • Information by UniProt
    • 数据库链接
    • 形式Localisation: Intermediate filament (Cytoskeleton).
    • 别名
      • CK 4 antibody
      • CK-4 antibody
      • CK4 antibody
      • CYK4 antibody
      • Cytokeratin 4 antibody
      • Cytokeratin-4 antibody
      • Cytokeratin4 antibody
      • FLJ31692 antibody
      • K2C4_HUMAN antibody
      • K4 antibody
      • Keratin 4 antibody
      • Keratin antibody
      • Keratin type II cytoskeletal 4 antibody
      • Keratin-4 antibody
      • Keratin4 antibody
      • KRT 4 antibody
      • Krt4 antibody
      • type II cytoskeletal 4 antibody
      • Type-II keratin Kb4 antibody
      see all

    Anti-Cytokeratin 4 antibody [EPR1598] 图像

    • All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Human uterus cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 57 kDa
    • ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
    • Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution.

    Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)参考文献

    This product has been referenced in:
    • Jacot TA  et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014). ICC ; Human . Read more (PubMed: 25489736) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab92465.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"