The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/2000. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).
Use at an assay dependent concentration.
组织特异性Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
疾病相关Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
Immunohistochemical analysis of paraffin embedded human transitional bladder carcinoma using ab51600 (1/100). Secondary staining with Goat anti-Rabbit HRP (1/2000).
Anti-Cytokeratin 4 antibody (ab51600)参考文献
This product has been referenced in:
Tabuchi Y et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim63:31-44 (2014).
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Vincent-Bugnas S et al. EBV infection is common in gingival epithelial cells of the periodontium and worsens during chronic periodontitis. PLoS One8:e80336 (2013).
Read more (PubMed: 24367478) »