重组Anti-Cytokeratin 3/CK-3抗体[AE5] (ab68260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 3/CK-3抗体[AE5]
参阅全部 Cytokeratin 3/CK-3 一抗 -
描述
小鼠单克隆抗体[AE5] to Cytokeratin 3/CK-3 -
宿主
Mouse -
特异性
This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
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经测试应用
适用于: IHC-Pmore details
不适用于: Flow Cyt (Intra),ICC,IP or WB -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC: Human squamous papilloma of cornea tissue
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常规说明
This product has switched from a hybridoma to recombinant production method on 5th January 2022
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AE5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68260于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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组织特异性
Cornea specific. -
疾病相关
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
序列相似性
Belongs to the intermediate filament family. - Information by UniProt
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数据库链接
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- Unigene: 680652 Human
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别名
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
图片
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All lanes : Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector containing a His tag®, whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with Cytokeratin full length expression vector containing a His tag®, whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
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Immunohistochemical analysis of paraffin-embedded human squamous papilloma of cornea tissue labelling Cytokeratin 3 with ab68260 at 1/500 dilution, followed by a ready-to-use secondary antibody. Cytosolic staining on human squamous papilloma of cornea tissue is observed. Counterstained with Hematoxylin. Positive staining on human squamous papilloma of cornea. The section was incubated with ab68260 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab68260 被引用在 27 文献中.
- Jaffet J et al. Clinical, histological and immunohistochemistry characteristics of cornea in the sequelae stage of chronic vernal keratoconjunctivitis. Indian J Ophthalmol 70:59-64 (2022). PubMed: 34937208
- Park SK et al. Hyaluronic acid hydrogels crosslinked via blue light-induced thiol-ene reaction for the treatment of rat corneal alkali burn. Regen Ther 20:51-60 (2022). PubMed: 35402662
- Yu Z et al. A human cornea-on-a-chip for the study of epithelial wound healing by extracellular vesicles. iScience 25:104200 (2022). PubMed: 35479406
- Eriksen AZ et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids. STAR Protoc 3:101383 (2022). PubMed: 35664254
- Jumelle C et al. Growth factor-eluting hydrogels for management of corneal defects. Mater Sci Eng C Mater Biol Appl 120:111790 (2021). PubMed: 33545916