Anti-Cytokeratin 3抗体[AE5] (ab77869)

概述

  • 产品名称Anti-Cytokeratin 3抗体[AE5]
    参阅全部 Cytokeratin 3 一抗
  • 描述
    小鼠单克隆抗体[AE5] to Cytokeratin 3
  • 经测试应用适用于: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Rabbit, Cow, Human
  • 免疫原

    Rabbit corneal epithelial keratins

性能

应用

Our Abpromise guarantee covers the use of ab77869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/3000. Predicted molecular weight: 65 kDa.
Flow Cyt Use 2µg for 106 cells.

靶标

  • 组织特异性Cornea specific.
  • 疾病相关Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
  • 序列相似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 65 kDa cytokeratin antibody
    • CK-3 antibody
    • CK3 antibody
    • Cytokeratin-3 antibody
    • K2C3_HUMAN antibody
    • K3 antibody
    • Keratin 3 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 3 antibody
    • Keratin-3 antibody
    • KRT3 antibody
    • type II cytoskeletal 3 antibody
    • Type-II keratin Kb3 antibody
    see all

Anti-Cytokeratin 3 antibody [AE5] 图像

  • Overlay histogram showing A431 cells stained with ab77869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77869, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab77869 staining Cytokeratin 3 (red) in transfected Ptk2 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were co-transfected in quadruplicate with wild-type, mutant, or a 1:1 mixture of wild-type and mutant K12 expression plasmids along with a wild-type K3 expression construct. After 72 hours, cells were fixed with 3% paraformaldehyde for 20 minutes, before being incubated with primary antibody. Nuclei were stained with DAPI (blue).

Anti-Cytokeratin 3 antibody [AE5] (ab77869)参考文献

This product has been referenced in:
  • Hanson C  et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol : (2012). Read more (PubMed: 22280565) »
  • Liao H  et al. Development of Allele-Specific Therapeutic siRNA in Meesmann Epithelial Corneal Dystrophy. PLoS One 6:e28582 (2011). ICC/IF ; Human . Read more (PubMed: 22174841) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us and your interest in our products.


As discussed over the phone, the Anti-Cytokeratin 3 antibody [AE5](ab77869) should be suitable for the experiment you are hoping to perform. This particular clone has been u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (tooth)
Specification tooth
Permeabilization Yes - triton x-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 30 2013

Thank you for your inquiry and your interest in our products.

I searched our catalogue and we currently do not have a cytokeratin 3 antibody that is directly conjugated. We do carry ab77869, which has been tested in FC with rabbit, cow and hu...

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Thank you for contacting Abcam.

I will be happy to help you further in this matter, but I just have a few more questions for you:

1 - Were you staining tissue sections or a monolayer of cultured cells?

2 - If you were stai...

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Thats no problem. If you have any further questions please do not hesitate to contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"