Anti-Cytokeratin 2e抗体[Ks2.342.7.1] (ab19122)

概述

  • 产品名称Anti-Cytokeratin 2e抗体[Ks2.342.7.1]
    参阅全部 Cytokeratin 2e 一抗
  • 描述
    小鼠单克隆抗体[Ks2.342.7.1] to Cytokeratin 2e
  • 特异性This antibody is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The antibody does not react with palate cytokeratin 2p.
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide:

    SCQISCKSRG RGGGGGGFRG FS

    , corresponding to N terminal amino acids 2 - 23 of Human Keratin 2e.

  • 阳性对照
    • IHC-P: FFPE human skin tissue sections.
  • 常规说明


    This antibody represents an excellent marker to study terminal epidermal differentiation.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: Tissue culture supernatant
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody represents an excellent marker to study terminal epidermal differentiation.
  • 克隆单克隆
  • 克隆编号Ks2.342.7.1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20.

靶标

  • 相关性Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • CK 2e antibody
    • Cytokeratin-2e antibody
    • Epithelial keratin-2e antibody
    • K2e antibody
    • Keratin type II cytoskeletal 2 epidermal antibody
    • Keratin-2 epidermis antibody
    • KRT2 antibody
    • KRT2A antibody
    • KRT2E antibody
    • Type-II keratin Kb2 antibody
    see all

Anti-Cytokeratin 2e antibody [Ks2.342.7.1] 图像

  • IHC image of Cytokeratin 2e staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19122, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cytokeratin 2e antibody [Ks2.342.7.1] (ab19122)参考文献

ab19122 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"